Basic Immunology (Amy Hahn - 1 hour)

• Innate immunity mediators
• Adaptive immunity
• Antibody structure, genetics, function
• T cell receptor structure, genetics, function
• MHC structure, polymorphism, inheritance
• Antigen processing and presentation
• T cell maturation, activation, effector functions
• B cell development, activation 
• Antibody response - humoral immunity
• Natural killer cell receptors, function

Molecular Genetics (Robert Liwski - 1 hr)

• DNA Structure
• RNA Structure and Function
• Transcription and Translation
• DNA Replication
• MHC Gene Organization

Transplant Immunology (Kelley Hitchman - 1 hour)

• Innate and adaptive mechanisms of rejection
• KIR
• Graft vs. Host Disease (GVHD)
• ABO
• Sensitization
• Preventative and therapeutic immunomodulation (induction, maintenance, desensitization) Immune memory/anamnestic responses

Molecular Typing (Michael Gautreaux - 1 hr)

• Facilities
• DNA extraction
• Forward and Reverse SSOP
• Sequence-specific Primers
• Quantitative PCR/Real Time-PCR
• Sanger Sequencing-Based Typing
• QC & Troubleshooting

Alleles, Epitopes, Haplotypes & Disease Associations (Amy Hahn - 1 hr)

• History of HLA
• Protein and molecular terminology
• CREGS, epitopes, eplets
• Serology typing and crossreactivity
• Polymorphism
• Linkage Disequilibrium, haplotypes
• Naming of HLA antigens/alleles
• Null alleles, G & P groups, NMDP codes
• Disease Associations
• HLA-associated drug hypersensitivity

Solid Organ Transplantation (Michael Gautreaux - 1 hr)

• History of transplantation
• Kidney transplantation overview
• Other abdominal transplantation
• Thoracic transplantation
• Vascularized composite allografts
• Organ allocation/UNOS/OPTN
• Rejection
• Xenotransplantation

Hematopoietic Stem Cell Transplantation (Robert Liwski - 1 hr)

• Autologous HSCT
• Syngeneic HSCT
• Allogeneic HSCT
• HSC Sources
• Donor Selection

Quality Process & Regulatory Compliance (Walter Herczyk and Arianne Mankey)

• Laboratory Quality Control
• Laboratory Quality Assessment
• Quality Improvement Plans
• Laboratory Regulatons/Compliance
• Laboratory Safety

Antibody Detection by Flow Cytometry/Luminex (Kelley Kitchman - 1 hour)

• Principles of Flow Cytometry
• Anti-HLA antibodies and transplantation
• Evolution of antibody testing methods
• Common current antibody testing methods
• Sample consderations (serum selection, sample quality, timing, interference)
• Analytics and interpretation 
• Pre-transplant vs. Post-transplant (DSA) considerations
• Troubleshooting

Crossmatching for Transplantation (Michael Gautreaux - 1 hr)

• Complement-Dependent Cytotoxicity
• Flow Cytometric Crossmatching
• Virtual Crossmatch
• Renal vs. Non-Renal Crossmatching